Service steps

Services

Deliverables

Delivery period

Vector construction

(1) Gene synthesis and codon optimization (optional)

(2) Fragment construction into an appropriate expression vector

(1) Expression plasmid 1-5 ug

(2) Expression Plasmid Sequencing Report

(3) Protein product of 0.1-5 mg, with a purity >85%.

(4) Protein Product Report

1 - 3 weeks

Cell transfection

(1) Plasmid transfected cells

(2) Optimization of fermentation conditions for expression test

(3) Purification of laboratory-scale samples

1 - 2 weeks

Amplification and fermentation

(1) Amplification and fermentation

(2) Protein purification

(3) Protein Test Report

1 - 2 weeks

Service steps

Services

Deliverables

Delivery period

Vector construction

(1) Gene synthesis and codon optimization (optional).

(2) Fragment construction into the transfer vector.

(3) Transfer vector recombination and recombinant bacmid extraction

(1) Expression plasmid 1-5 ug

(2) Expression Plasmid Sequencing Report

(3) Recombinant virus

(4) Protein product of 0.1-5 mg, with a purity >85%.

(5) Protein Product Report

2 - 3 weeks

Recombinant virus and amplification

(1) Recombinant bacmid transfected cells

(2) Recombinant virus amplification

(3) Laboratory-scale fermentation and purification

1 - 3 weeks

Amplification and fermentation

(1) Amplification and fermentation

(2) Protein purification

(3) Protein Test Report

1 - 2 weeks

Service steps

Services

Deliverables

Delivery period

Vector construction

(1) Gene synthesis and codon optimization (optional)

(2) Fragment construction into an appropriate expression vector

(1) Expression plasmid 1-5 ug

(2) Expression Plasmid Sequencing Report

(3) Positive Strain Screening Report

(4) Optimal expression bacteria

(5) Laboratory-scale expression results

(6) Purification of 0.1-1 mg of target protein

(7) COA of Protein Product

1 - 3 weeks

Single colony screening

(1) Vector linearization

(2) Vector electrotransformation of Pichia pastoris cells

(3) PCR screening of positive transformants

1 - 3 weeks

Laboratory-scale expression

(1) Four positive clones ed for expression Quantity of laboratory-scale validation

(2) Expression analysis validation (WB)

Optional: High-copy strain screening

1 - 3 weeks

Amplification and fermentation

(1) Amplification and fermentation

(2) Protein purification

(3) Protein Test Report

2 - 4 weeks

Service steps

Services

Deliverables

Delivery period

Vector construction

(1) Gene synthesis and codon optimization (optional)

(2) Fragment construction into an appropriate expression vector

Note: Label-free, HIS label, sumo, GST, TRXA and MBP are optional

(1) 1-5 ug liquid plasmid of expression vector plasmid

(2) Protein product of 1-5 mg, with a purity >85%

(3) Protein Product Report

1 - 2 weeks

Expression test

(1) Test expression of small volume fermentation (soluble/inclusion body expression)

(2) Small volume purification test

(3) Other options: label removal, inclusion body purification

1 - 3 weeks

Delivery and reporting

(1) Delivery of protein

(2) Protein Test Report

<1 week